A Facile and Modified Scheme for Synchronization and Isolation of Nematode Eggs

Nematodes are common pests that damage agricultural crop plants. Some of them beneficial while others parasitic and harmful to plants, animals humans. Several in vitro studies have aimed develop chemicals kill nematodes, been conducted use nematodes as biocontrol agents. However, the preparation large quantities nematode eggs a laboratory setting is challenging. Traditional egg isolation protocols involve sieves filter or can be isolating eggs. Our method utilizes 1.5 × cm sized chunks bacterial fungal feed lure nematodes. A subsequent gentle washing consumed chunk with distilled water provides rapid straightforward collecting 6-well polystyrene plates removing unwanted Approximately 4000 Bursaphelenchus xylophilus from 2400 Caenorhabditis elegans were obtained when tested. This study shows protocol for synchronization stages relatively straightforward, rapid, eco-friendly, efficient. The also chemical-free reliable, simple means separating adults induces based on concept gravid provoked lay by providing additional feed.